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anti mpc1  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc anti mpc1
    Anti Mpc1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+mpc1/pmc12989855-5-0-5
    Average 86 stars, based on 1 article reviews
    anti mpc1 - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Staining:

    Article Title: Long-Term Effects of Radiation Therapy on Cerebral Microvessel Proteome: A Six Month Post-Exposure Analysis
    Article Snippet: Equal amounts of protein (5–10 μg) from control and irradiated groups were separated on NuPAGE 4% to 12% Bis-Tris gels (Life Technologies) and transferred to polyvinylidene difluoride (PVDF) membranes using the Mini-PROTEAN 3 blotting system (Bio-Rad). .. Membranes were stained with Ponceau S (Cell Signaling #59803S) to confirm protein transfer, washed in TBS with Tween (TBST), and blocked with 3% BSA (RPI Research #9048–46–8) or Starting Block Blocking Buffer (Thermo Fisher Scientific #37538) for 2 h. Membranes were incubated overnight at 4 °C with primary antibodies, including anti-NDUFB8 (Abcam, no. ab110413), anti-NDUFA11 (ABclonal, no. A16239), anti-NDUFA4 (ABclonal, no. A15693), anti-SDHA (Cell Signaling, no. 11998), anti-SDHB (Abcam, no. ab110413), anti-UQCRC2 (Aviva systems biology, no. OAAN01132), anti-MT-CO2 (ABclonal, no. A11154), anti-COX6B1 (ABclonal, no. A2228), anti-ATP5F1C (ABclonal, no. A15257), anti-ATP6VID (ABclonal, no. A12940), anti-ATP5F1A (ABclonal, no. A11217), anti-ACLY (ABclonal, no. A3719), anti-IDH3A (ABclonal, no. A14650), anti-DLD (ABclonal, no. A13296), anti-ACO2 (ABclonal, no. A3716), anti-MDH2 (ABclonal, no. A13516), anti-ENO2 (Cell Signaling, no. 9536), anti-TPI (ABclonal, no. A15733), anti-MPC1 (Cell Signaling, no.14462), and anti-MPC2 (Cell Signaling, no. 46141). ..

    Blocking Assay:

    Article Title: Long-Term Effects of Radiation Therapy on Cerebral Microvessel Proteome: A Six Month Post-Exposure Analysis
    Article Snippet: Equal amounts of protein (5–10 μg) from control and irradiated groups were separated on NuPAGE 4% to 12% Bis-Tris gels (Life Technologies) and transferred to polyvinylidene difluoride (PVDF) membranes using the Mini-PROTEAN 3 blotting system (Bio-Rad). .. Membranes were stained with Ponceau S (Cell Signaling #59803S) to confirm protein transfer, washed in TBS with Tween (TBST), and blocked with 3% BSA (RPI Research #9048–46–8) or Starting Block Blocking Buffer (Thermo Fisher Scientific #37538) for 2 h. Membranes were incubated overnight at 4 °C with primary antibodies, including anti-NDUFB8 (Abcam, no. ab110413), anti-NDUFA11 (ABclonal, no. A16239), anti-NDUFA4 (ABclonal, no. A15693), anti-SDHA (Cell Signaling, no. 11998), anti-SDHB (Abcam, no. ab110413), anti-UQCRC2 (Aviva systems biology, no. OAAN01132), anti-MT-CO2 (ABclonal, no. A11154), anti-COX6B1 (ABclonal, no. A2228), anti-ATP5F1C (ABclonal, no. A15257), anti-ATP6VID (ABclonal, no. A12940), anti-ATP5F1A (ABclonal, no. A11217), anti-ACLY (ABclonal, no. A3719), anti-IDH3A (ABclonal, no. A14650), anti-DLD (ABclonal, no. A13296), anti-ACO2 (ABclonal, no. A3716), anti-MDH2 (ABclonal, no. A13516), anti-ENO2 (Cell Signaling, no. 9536), anti-TPI (ABclonal, no. A15733), anti-MPC1 (Cell Signaling, no.14462), and anti-MPC2 (Cell Signaling, no. 46141). ..

    Article Title: Oral Sulforaphane Intervention Protects Against Diabetic Cardiomyopathy in db/db Mice: Focus on Cardiac Lipotoxicity and Substrate Metabolism
    Article Snippet: Equal amounts of protein (15–20 μg) were resolved by 10–15% SDS–PAGE gels and then transferred onto polyvinylidene fluoride (PVDF) membranes (Millipore, Billerica, MA, USA). .. After blocking with 5% nonfat milk for 2 h, the following primary antibodies were used: Anti-CD36 (1:1000, Abcam, ab200353), Anti-H-FABP (1:2000, Abcam, ab133585), Anti-FATP4 (1:1000, Abcam, ab252923), Anti-CPT1B (1:1000, Abclonal Technology, A6796), Anti-PPARα (1:1000, Santa Cruz Biotechnology, sc-398394), Anti-GLUT4 (1:2000, Santa Cruz Biotechnology, sc-53566), Anti-PDK4 (1:1000, Abclonal Technology, A13337), Anti-MPC1 (1:1000, Cell Signaling Technology, 14462S), Anti-MPC2 (1:1000, Cell Signaling Technology, 46141S), and Anti-β-actin (1:1000, Santa Cruz Biotechnology, sc-47778). .. The immunoreactive bands were detected using horseradish peroxidase-conjugated secondary antibodies (Santa Cruz Biotechnology, Dallas, TX), and visualized using the ECL detection kit (Thermo Fisher, San Jose, CA, USA).

    Article Title: Oral Sulforaphane Intervention Protects Against Diabetic Cardiomyopathy in db/db Mice: Focus on Cardiac Lipotoxicity and Substrate Metabolism.
    Article Snippet: Equal amounts of protein (15–20 μg) were resolved by 10–15% SDS–PAGE gels and then transferred onto polyvinylidene fluoride (PVDF) membranes (Millipore, Billerica, MA, USA). .. After blocking with 5% nonfat milk for 2 h, the following primary antibodies were used: Anti-CD36 (1:1000, Abcam, ab200353), Anti-H-FABP (1:2000, Abcam, ab133585), Anti-FATP4 (1:1000, Abcam, ab252923), Anti-CPT1B (1:1000, Abclonal Technology, A6796), Anti-PPARα (1:1000, Santa Cruz Biotechnology, sc-398394), Anti-GLUT4 (1:2000, Santa Cruz Biotechnology, sc-53566), Anti-PDK4 (1:1000, Abclonal Technology, A13337), Anti-MPC1 (1:1000, Cell Signaling Technology, 14462S), Anti-MPC2 (1:1000, Cell Signaling Technology, 46141S), and Anti-β-actin (1:1000, Santa Cruz Biotechnology, sc-47778). .. The immunoreactive bands were detected using horseradish peroxidase-conjugated secondary antibodies (Santa Cruz Biotechnology, Dallas, TX), and visualized using the ECL detection kit (Thermo Fisher, San Jose, CA, USA).

    Incubation:

    Article Title: Long-Term Effects of Radiation Therapy on Cerebral Microvessel Proteome: A Six Month Post-Exposure Analysis
    Article Snippet: Equal amounts of protein (5–10 μg) from control and irradiated groups were separated on NuPAGE 4% to 12% Bis-Tris gels (Life Technologies) and transferred to polyvinylidene difluoride (PVDF) membranes using the Mini-PROTEAN 3 blotting system (Bio-Rad). .. Membranes were stained with Ponceau S (Cell Signaling #59803S) to confirm protein transfer, washed in TBS with Tween (TBST), and blocked with 3% BSA (RPI Research #9048–46–8) or Starting Block Blocking Buffer (Thermo Fisher Scientific #37538) for 2 h. Membranes were incubated overnight at 4 °C with primary antibodies, including anti-NDUFB8 (Abcam, no. ab110413), anti-NDUFA11 (ABclonal, no. A16239), anti-NDUFA4 (ABclonal, no. A15693), anti-SDHA (Cell Signaling, no. 11998), anti-SDHB (Abcam, no. ab110413), anti-UQCRC2 (Aviva systems biology, no. OAAN01132), anti-MT-CO2 (ABclonal, no. A11154), anti-COX6B1 (ABclonal, no. A2228), anti-ATP5F1C (ABclonal, no. A15257), anti-ATP6VID (ABclonal, no. A12940), anti-ATP5F1A (ABclonal, no. A11217), anti-ACLY (ABclonal, no. A3719), anti-IDH3A (ABclonal, no. A14650), anti-DLD (ABclonal, no. A13296), anti-ACO2 (ABclonal, no. A3716), anti-MDH2 (ABclonal, no. A13516), anti-ENO2 (Cell Signaling, no. 9536), anti-TPI (ABclonal, no. A15733), anti-MPC1 (Cell Signaling, no.14462), and anti-MPC2 (Cell Signaling, no. 46141). ..

    Article Title: Long-Term Effects of Radiation Therapy on Cerebral Microvessel Proteome: A Six-Month Post-Exposure Analysis
    Article Snippet: Membranes were stained with Ponceau S (Cell Signaling #59803S) to confirm protein transfer, washed in TBS with TweenTM (TBST), and blocked with 3% BSA (RPI Research #9048-46-8) or Starting BlockTM Blocking Buffer (Thermo Fisher Scientific #37538) for 2 hours. .. Membranes were incubated overnight at 4°C with primary antibodies, including anti-NDUFB8 (Abcam, no. ab110413), anti-NDUFA11 (ABclonal, no. A16239), anti-NDUFA4 (ABclonal, no. A15693), anti-SDHA (Cell Signaling, no. 11998), anti-SDHB (Abcam, no. ab110413), anti-UQCRC2 (Aviva systems biology, no. OAAN01132), anti-MT-CO2 (ABclonal, no. A11154), anti-COX6B1 (ABclonal, no. A2228), anti-ATP5F1C (ABclonal, no. A15257), anti-ATP6VID (ABclonal, no. A12940), anti-ATP5F1A (ABclonal, no. A11217), anti-ACLY (ABclonal, no. A3719), anti-IDH3A (ABclonal, no. A14650), anti-DLD (ABclonal, no. A13296), anti-ACO2 (ABclonal, no. A3716), anti-MDH2 (ABclonal, no. A13516), anti-ENO2 (Cell Signaling, no. 9536), anti-TPI (ABclonal, no. A15733), anti-MPC1 (Cell Signaling, no.14462), and anti-MPC2 (Cell Signaling, no. 46141). ..

    Article Title: Astrocyte MCT1 Expression Does Not Contribute to the Axonal Degenerative Phenotype Observed With Ubiquitous MCT1 Depletion
    Article Snippet: After electrophoresis, proteins were blotted on a PVDF membrane (Biorad) and blocked in 5% Blotting Grade Blotter (Biorad) diluted in Tris buffered saline (TBS) containing 0.1% Tween 20 (TBST). .. Blots were then incubated with one of the following antibodies: mouse anti‐PLP/DM20 (MilliporeSigma), chicken anti‐human MCT1 antibody (our lab), mouse anti‐GAPDH (Thermo Scientific), rabbit anti‐MPC1 (Cell Signaling), rabbit anti‐MPC2 (Cell Signaling), and mouse anti‐alpha tubulin (Abcam). ..

    Cell-Signaling:

    Article Title: Disturbed mitochondrial maturation in cardiolipin remodeling-deficient cardiomyocytes
    Article Snippet: anti-CPT2, rabbit monoclonal ( EPR13626 ) , Abcam , Cat#ab181114; RRID: AB_2687503. .. anti-MPC1, rabbit monoclonal (D2L9I) , Cell Signaling Technology , Cat#14462; RRID: AB_2773729. .. anti-MPC2, rabbit monoclonal (D417G) , Cell Signaling Technology , Cat#46141; RRID: AB_2799295.

    Western Blot:

    Article Title: The fate of pyruvate dictates cell growth by modulating cellular redox potential
    Article Snippet: Antibody , Cy3 conjugated anti-mouse (host: donkey polyclonal) , Jacksons Immuno Research Laboratories , Cat# 115-165-166 , Dilution factor 1:400 for immunofluorescence. .. Antibody , anti-MPC1 (host: rabbit monoclonal) , Cell Signaling Technologies , Cat# 14462 , Dilution factor 1:1000 for western blot. .. Antibody , anti-MPC2 (host: rabbit monoclonal) , Cell Signaling Technologies , Cat# 46141 , Dilution factor 1:1000 for western blot.



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